Computational and Experimental Investigation of the Combined Effect of Various 3D Scaffolds and Bioreactor Stimulation on Human Cells’ Feedback

Computational methods were combined with an experimental setup in order to investigate the response of human umbilical cord stem cells 3D electrospun and printed scaffolds, when dynamically stimulated a bioreactor. Key parameters associated bioreactor working conditions computationally investigated using Comsol software use output for planned setup. Based on theoretical observations, influence inlet velocity, cell number, exposure time analyzed vitro adjusted accordingly. MSCs seeded different numbers porous scaffolds (0.5 2 h duration, 3 6 mm/s velocity). Polycaprolactone electrospun, polyurethane polylactic acid 3D-printed fabricated fibronectin-coated. The computational study predicted initial events process deposition attachment. Total protein, osteopontin, osteocalcin levels deposited investigated; SEM confocal imaging confirmed biomarker analysis. proliferated well PCL. Polyurethane enabled extremely rapid proliferation followed by differentiation, while PLA induced moderate parallel mineralization. stiffness has been found as key enabling parameter decisive feedback.